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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Runx3 Induces a Cell Shape Change and Suppresses Migration and Metastasis of Melanoma Cells by Altering a Transcriptional Profile
doi: 10.3390/ijms22042219
Figure Lengend Snippet: Microarray analysis uncovered an altered and specific transcriptional profile underlying the cell shape change and the suppression of the metastatic potential by Runx3. ( A ) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations assigned different repertoires of differentially expressed genes (DEGs) that were regulated by Runx3 in B16-F10 cells and, in the case of B16-F0 cells vs. B16-F10 cells, to the terms relevant to the actin cytoskeleton and adhesion. Each GO/KEGG annotation term is inscribed as a caption. The number of DEGs within a repertoire is described at x axis. The columns for the downregulated and the upregulated DEGs are shown in different colors as explained at the top of the figure., Down/Up by Runx3 indicates the appearance of DEGs in the case of B16-F10/Runx3 cells vs. mock control B16-F10 cells. Down/Up in B16-F0 indicates the appearance of DEGs in the case of B16-F0 cells vs. B16-F10 cells. Down/Up in common indicates the appearance of same DEGs in both cases. ( B ) Runx3 upregulated the expression of extracellular matrix (ECM) genes. The summary table indicates the gene names and their corresponding fold changes. ( C ) Runx3 regulated the expression of a list of DEGs that were inversely associated with an increase in the metastatic potential of B16-F10 cells compared to B16-F0 cells. The heatmap shows the gene names and their fold changes in the cases of B16-F10/Runx3 cells vs. mock control B16-F10 cells and B16-F0 cells vs. B16-F10 cells, respectively.
Article Snippet:
Techniques: Microarray, Control, Expressing
Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays
doi: 10.1007/978-1-4939-6584-7_11
Figure Lengend Snippet: Overview of SMM strategy to identify RNA-binding small molecules. Small molecule libraries prepared in 384-well plates are utilized to print onto isocyanate-functionalized slides by the microarrayer. Prepared SMMs are next incubated with the labeled RNA of interest and scanned to detect hits
Article Snippet: Printing of
Techniques: RNA Binding Assay, Incubation, Labeling